Human Argonaute 2 is a programmable targeting domain for RNA editing

19659001 ALTER is a programmable RNA base editor developed totally from human proteins. By utilizing human AGO2 rather of bacterial CRISPR– Cas proteins, ALTER alleviates a crucial threat element for hazardous immune reactions in healing applications while likewise showing normally more efficient than dCas13-based editors. This is a sneak peek of membership material, gain access to through your organization 19659003 Gain access to alternatives Gain access to through your organization 19659004 19659005 Gain access to Nature and 54 other Nature Portfolio journals Get Nature+, our best-value online-access membership 19659007 $32.99 19459256/ 30 days 19659008 cancel at any time Find out more Register for this journal Get 12 print concerns and online gain access to $259.00 19459256 annually just $21.58 per problem 19659014 Discover more 19659015 Purchase this short article 19659016 Purchase on SpringerLink Immediate access to the complete short article PDF. 19659018 USD 39.95 19659019 Costs might undergo regional taxes which are determined throughout checkout 19659020 19659021

Fig. 1: ALTER. 19459279 Topics 19659024 References 19459253 Charlesworth, C. T. et al. Recognition of preexisting adaptive resistance to Cas9 proteins in human beings. 19459287 Nat. Medication. 19659025 25 , 249– 254(2019). This research study determined the high frequency of anti-Cas antibodies and T cell actions in people. Article 19459251 CAS PubMed Central Google Scholar 19659027 Iwakawa, H. & Tomari, Y. Life of RISC: development, action, and deterioration of RNA-induced silencing complex. Mol. Cell 82 , 30– 43( 2022 ). 19459289 An evaluation on AGO2 function in RNAi that covers all phases of the procedure. Article CAS PubMed 19459251 19459038 data-track-action=19459064 data-track-value=19459064 data-track-label=data-track-item_id=rel=19459039 aria-label=href=http://19459097> Google Scholar Becker, W. R. et al. High-throughput analysis exposes guidelines for target RNA binding and cleavage by AGO2. Mol. Cell 75 , 741– 755. e11(2019). This research study included massive screening of how various gRNA qualities effect AGO2 binding and target cleavage. Article 19459251 CAS PubMed 19459251 PubMed Central 19459251 Google Scholar Huang, X. et al. Programmable C-to-U RNA modifying utilizing the human APOBEC3A deaminase. EMBO J. 19659034 39 , e104741(2020). 19459289 The research study explains the engineering of CURE editors. 19659035 Article 19459251 CAS PubMed 19459251

PubMed Central 19459251 Google Scholar 19659036 Wolfsberg, E. et al. Machine-guided dual-objective protein engineering for deimmunization and restorative functions. Cell Syst. 19659037 16 19459270, 101299 (2025). An AI-assisted workflow for deimmunization of crafted protein blends. 19659038 Article CAS PubMed PubMed Central 19459251 Google Scholar 19459251 19659039 Download referrals 19659040

Additional details 19659041 Publisher’s note 19459270 Springer Nature stays neutral with regard to jurisdictional claims in released maps and institutional associations. 19659042 This is a summary of: Lusi, R. F. et al. Engineered human Argonaute 2 for targeted C-to-U RNA base modifying. 19459287 Nat. Biotechnol https://doi.org/10.1038/s41587-026-03359-y 19459251 (2026). 19659043

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About this post 19659045 Mention this post 19659046 Human Argonaute 2 is a programmable targeting domain for RNA modifying. Nat Biotechnol (2026). https://doi.org/10.1038/s41587-026-03358-z 19659047 Download citation 19659048 19659049 Released : 19659050 22 October 2026 19659051 Variation of record 19459256: 19659052 22 October 2026 DOI : https://doi.org/10.1038/s41587-026-03358-z